Cellular Micropatterns with Built-in Biosensors for Detection of Extracellular Metabolites
نویسندگان
چکیده
We developed a strategy for integrating cells and biosensors based on nonfouling poly (ethylene glycol) (PEG) hydrogel microstructures serving a dual role of cell culture chambers and matrices for encapsulation of sensing elements. We demonstrated incorporation of biosensors into micropatterned mono and co-cultures, and sucessfully detected release of endogenous hydrogen peroxide (H2O2) by macrophages. This strategy for integrating cells and miniature sensing elements may be used in the future for detection of multiple metabolites from a small group of cells.
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